FP6 - 512009 eBIOSENSE - Electrical Bio Sensor Arrays for Analyses of Harmful Micro Organisms and Microbial Toxins

Contacts
Website: www.biotech.kth.se/eBIOSENSE




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Biotechnology : Life Science and Food Quality

Type of Project	STREP
Contract No	FP6 - 512009
Total Cost	3,380 KEuro
EC Contribution	2,370 KEuro
Start Date	1-1-2005
Duration	36 Months

Abstract

The amoin objective of this project is to develop an advanced platform for analysis of harmful micro organisms and/or their toxic products. The goal will be reached by integration of the data on genomes and proteomes available through bioinformatics and advanced silicon and microsystem technology.

The platform, based on ambitious electric biochip arrays, will enable parallel and simultaneous identification and quantification of specific nucleic acids, microbial proteins and toxic microbial products. The targets are recognized and captured by DNA probes or antibodies on the electrochemical transducer surface, that are arrays of nm-sized interdigital gold electrodes The captured analyte molecules are labelled with an enzyme that produces an electroactive product thus generating an electric current that corresponds to the concentration of the target analyte in the sample.

All application developing partners use this platform and same electrical read out instrumentation but they implement their special expertise into development of specific innovative chip arrays. The DNA-and protein-arrays require different and specific procedures for chip loading of the recognition elements, and for detection.

The final instruments will be portable devices and are furnished with disposable chips. The technique will be generic but the applications in the project are selected analyses of food and water born harmful micro organisms and microbial toxins:

• Shiga toxin producing Escherichia coli (STEG, ETEC, EHEC etc);
• Salmonella enteriditis
• pathogenic strains of Bacillus cereus
• pathogenic Staphylococcus strains that cause food poisoning and infections,
• Legionella responsible for the Legionnaire's disease distributed via water, and
• Mycotoxins.

The diagnostic equipment is being evaluated for functionality in different exploratory prototypes by a large industrial partner and a specialised SME, assessing the quality management issues for pharma/food/evironmental industry.

Coordinator

Kungl. Tekniska Hogskolan, School of Biotechnology, Royal Institute of Technology, Sweden

Partners

• Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V., Institute for Silicon Technology, Germany
• Ernst Moritz Arndt Universität, Greifswald, Germany
• University of Oulu, Department of Process and Environmental Engineering, Finland
• University of Gdansk, Department of Molecular Biology, Poland
• eBiochip Systems GmbH, Germany
• Scanbec Oy, Finland
• Plant Research International b.v., Netherlands
• Centre de Recherche et d'étude pour l'alimentation, France
• Raisio Benecol Ltd., Finland

Contacts

Coordinator

• Sven-Olof ENFORS / Royal Institute of Technology Dept. of Biotechnology / SWEDEN