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[BioMatNet Database - FAIR Program] FAIR-CT95-1120
Rapid detection systems for mould contamination
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Biotechnology : FAIR Area 3 - Generic Science and Advanced Technologies for Nutritious Foods : Integrated Crop Protection & Biological Control



Proposal No: FAIR-CT95-1120
Date Prepared: January 1998
Source: Proposal Abstract

Abstract:
Fungal contamination of food and feeds causes considerable economic losses as well as posing a health threat to the consumer, because of mycotoxin production. Current methods for detection of fungal infection in foods are time-consuming and insensitive and do not make use of recent technological developments in analytical methods. The proposal combines the complementary expertise of four research groups in Europe in a coordinated assay development and validation programme. The primary objective of the project is to develop and validate rapid and convenient broad-specificity assays for general fungal contamination of cereals, which will be applicable to both laboratory and field use. The emphasis will be place on immunoassays based on broad-spectrum antibodies to fungal mycelial proteins and also antibodies to ergosterol, a fungal sterol. Instrumental methods (NIR/NIT, electronic nose) for ergosterol detection will also be examined and all methods compared with traditional methods. The study will give a useful insight into the antigenic composition and molecular characterisation of common fungal contaminants of cereals, which will contribute to the setting up of speedier classification systems for novel fungal species.





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