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QLK3-2001-00403
Whole-cell Baeyer-Villiger monooxygenase biocatalysts: Methodologies for exploitation (BIOMEX)
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| Type of Project |
Research Project |
| Contract No |
QLK3-2001-00403 |
| Total Cost |
|
| EC Contribution |
804,305 EUR |
| Start Date |
01-01-2002 |
| Duration |
24 Months |
Abstract
This project is aimed at developing and applying novel methodologies for the exploitation of
Baeyer-Villiger monooxygenase-based biocatalysts. In a Framework IV project
(BIO4-CT98-0267), it was shown that the use of these biocatalysts is possible on a laboratory
and pilot plant scale for the synthesis of optically pure lactones as useful synthetic reagents for a range
of industrial products. However, scale-up beyond this for industrial exploitation requires addressing a
number of key bottlenecks.
The aim of this project is to address these bottlenecks with the objective of devising safe,
efficient and environmentally compatible processes suitable for large-scale operation and to
demonstrate this with the industrial partner at large scale. Additionally, a systematic scale-up
methodology will be demonstrated.
Objectives
- to develop a methodology to scale-up Baeyer-Villiger based biocatalytic processes in an
effective manner, including the use of mathematical modelling informed by economic and environmental
constraints.
- to develop solutions to the key process bottlenecks limiting application, including effective
biocatalyst production, extended biocatalyst activity/reuse and novel techniques to afford effective
downstream processing for product recovery and biocatalyst recycle.
- to demonstrate the efficient operation of Baeyer-Villiger based biocatalysts at large scale for a
limited number of products.
Activities
It is proposed to develop methods for biocatalytic process development and apply these to
Baeyer-Villiger based biocatalysis. The approach taken will be:
- collect the necessary process data on a laboratory scale to obtain parameters for process
modelling.
- identify the limitations to the existing process using process models. Examine the cost-benefit
analysis of alternative solutions to enable adequate scale-up for commercial competitiveness. Analyse
environmental constraints using these models.
- identify solutions to the key process bottlenecks and evaluate these at a laboratory scale.
- devise a base process based on the findings in (3).
- demonstrate the process at large scale using a limited number of representative substrates.
Deliverables
- methodology for modelling biocatalytic processes to identify bottlenecks due to economic
and environmental constraints.
- process solutions to specific bottlenecks, including improved downstream processing,
enhanced biocatalyst life and effective biocatalyst production.
- identifiable generic processes for immediate commercialisation, demonstrated at large scale,
for Baeyer-Villiger based biocatalysis.
Contacts
Coordinator
Participant
